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Insert the needle and let the water flow slowly down the vial wall

[PubMed: 16204362] 212

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During the formation of higher alcohols, the mitochondrial branched-chain isoleucine-leucine-valine (ILV) pathway metabolizes pyruvate into -ketoisovalerate through acetolactate synthase (Ilv2), acetohydroxyacid reductoisomerase (Ilv5) and dihydroxyacid dehydratase (Ilv3) (Kohlhaw 2003)
