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Proc Natl Acad Sci U S A 103:1376513770

1.1 Pathophysiology of tight junction proteins Tight junction (TJ) proteins connect adjacent epithelial cells on their apical side and therefore are critical for controlling paracellular permeability by selectively regulating the flow of ions, solutes, and small molecules across the epithelium

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The t of 46.3 h for the HBSS buffer (pH 7.4) also indicated the viable timeframes of the subsequent experiments that require physiological conditions, such as cell culture studies