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The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the antibody (R1401-12) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system

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Its use outside of a hospital setting is considered illegal and akin to dangerous drug usage, he added

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