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Thus, we buffer-exchanged the wild-type and the Q260A/R340A MraY into a buffer containing 0.05% LDAO (established as harsher, delipidating detergent than C8E4 or OG) 23 by size-exclusion chromatography and prepared aliquots containing a fixed protein concentration and an increasing C 55 -P concentration

It helps break down energy reserves stored in cells, making them available to muscle tissue, and consequently increases muscle endurance, so necessary during intense physical training

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4A) at the N-terminus, residue 16 to 36 (historical nomenclature, based on residue 1 occurring after signal peptide cleavage) is relatively long and is primarily responsible for lipoprotein binding